MSL973027 — Perform techniques that prevent cross-contamination
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What an assessment for MSL973027 must cover
49 assessable components: 3 elements (17 performance criteria), 17 performance evidence and 15 knowledge evidence requirements. An audit-defensible tool maps every question and task back to these — that mapping is the coverage matrix Auditori generates alongside the assessment.
Elements & performance criteria
1 Prepare for sampling or transfer
- 1.1Check the sampling procedure conforms with the required sampling plan
- 1.2Use specified personal protective equipment (PPE)
- 1.3Prepare the work area for safe and effective sample workflows
- 1.4Select equipment and materials specified by the procedure
- 1.5Organise equipment to minimise contamination during manipulations
- 1.6Label containers for clear identification
- 1.7Record details in relevant log or database
- 1.8Macroscopically assess media and/or reagents to confirm integrity before use
2 Transfer materials
- 2.1Protect the integrity of the sample source
- 2.2Perform transfer while minimising opportunities for contamination and/or cross-infection
- 2.3Protect the integrity of the sample source and destination after transfer, and before sealing the transport or culture vessel
- 2.4Perform quality control checks to confirm contaminate-free transfer was successful
- 2.5Label transport or culture vessels for clear identification
3 Maintain work area and equipment to prevent cross-infection and contamination
- 3.1Place disposable and reusable items into relevant receptacles
- 3.2Clean, disinfect and/or sterilise work area and equipment after use
- 3.3Transport disposable and reusable contaminated materials to relevant areas for disinfection, sterilisation and cleaning or disposal
- 3.4Follow personal hygiene procedures
Performance evidence
- safely and effectively performed techniques that prevent cross-contamination when performing at least 3 different types of sample transfers, with at least 3 from the following list:
- bacterial cultures
- body fluids (or simulated body fluids)
- sterile liquid culture media
- purified reagents
- water
- soil
- sterile pharmaceuticals
- fungi
- dairy product
- swabs
- smears
- propagation tissue
- plant material
- fermented foods
- fermented beverages
- Fresh animal tissue
Knowledge evidence
- principles of aseptic techniques
- growth requirements of microorganisms including bacteria and fungi in terms of their laboratory culture
- relationship between sterile practices, hygiene procedures and the ability to obtain samples free of contamination
- relationship between sterile practices and accurate test results
- importance of pure culture techniques and aseptic transfer to the successful microbiological investigation and correct interpretation of laboratory results
- cleaning and sanitising requirements of equipment and work area, and effects of physical and chemical agents on microbial growth and death
- requirements for handling specimens and processing samples for molecular analysis including nucleic acids and amplified specimens.
- principles of workflow isolation to minimise contamination
- sterilisation techniques, including: flaming instruments and vessel openings; high temperature, boiling and autoclaving; membrane filtration; radiation, gas and/or chemical treatments
- disinfection and sterilisation procedures used in the collection, processing and safe disposal of samples and materials
- principles of infection control related to work health and safety (WHS), and sampling and transfer of materials in microbiological investigations including how to minimise the generation of aerosols when flaming
- relevant hazards and how to deal with the risks presented, including: injuries from sharps, burners, molten agar; ultraviolet (UV) light sources; exposure to hazardous substances and/or infectious agents
- types and purpose of chemical and biological fume and safety cabinets
- environmental sustainability issues as they relate to the work task
- legal, ethical and WHS requirements specific to the work task.
Unit content sourced from training.gov.au — © Commonwealth of Australia, licensed under CC BY 4.0. Auditori is not affiliated with the Department of Employment and Workplace Relations.
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Questions about assessing MSL973027
What does an assessment tool for MSL973027 need to cover?
To satisfy the Principles of Assessment and Rules of Evidence, an assessment for MSL973027 needs to address all 49 unit components: 3 elements with 17 performance criteria, 17 performance evidence requirements, 15 knowledge evidence requirements, and the foundation skills. A coverage matrix mapping each question and task to these components is what an auditor looks for.
How does Auditori generate an assessment tool for MSL973027?
Auditori pulls the current release of MSL973027 from training.gov.au and generates a complete package: candidate assessment, assessor guide with model answers and observation criteria, and a coverage matrix mapping every component. A suitably qualified person then reviews and approves the draft in a built-in workflow — consistent with ASQA's guidance on AI use in VET — before export as branded PDF and editable Word.
Is the first assessment tool really free?
Yes. Every new account includes one free credit — enough to generate the complete assessment tool for MSL973027 — with no card and no subscription required. After that it's pay-as-you-go per unit.
Can I check my existing MSL973027 assessment instead of generating a new one?
Yes — upload your existing assessment or learner guide and Auditori maps it against every element, performance criterion, PE and KE of MSL973027, showing exactly what's covered and what's missing. Mapping costs a quarter of a credit.
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