MSL973015 — Prepare culture media

1 Prepare culture media

• 1.1Prepare mixture of media and solvent to ensure solution and even settling of heat soluble materials
• 1.2Label media to allow tracking in subsequent processes
• 1.3Use a vessel large enough to endure adequate mixing and heating of the media
• 1.4Check pH of media where pH is critical
• 1.5Dispense media into vessels for sterilisation, leaving room for expansion during heating and cooling

2 Sterilise media

• 2.1Load the steriliser in keeping with maximum permitted loads and appropriate positioning of materials
• 2.2Ensure a sterilisation indicator is correctly placed with the load to monitor sterilisation process
• 2.3Operate sterilisation cycle in accordance with manufacturer requirements to achieve sterilisation at the required settings
• 2.4Cool media to the temperature specified in the media formulation procedures
• 2.5Sterilise media and/or media additives using membrane filtration techniques

3 Dispense or aliquote media

• 3.1Add labile constituents where necessary, under conditions that will not lead to their denaturation or contamination of media
• 3.2Ensure even mixing of additives and media before dispensing
• 3.3Aseptically dispense media to minimise occurrence of procedural contamination
• 3.4Label media to allow for selection, avoiding areas of the culture vessel required for examination of colony growth

4 Label media

• 4.1Date batch media to ensure correct batch rotation
• 4.2Label media to allow for selection, avoiding areas of the culture vessel required for examination of colony growth
• 4.3Maintain records of batches of media

5 Store and incubate media

• 5.1Incubate control plates as a sterility check
• 5.2Store media to maximise shelf life and minimise contamination

6 Perform quality control checks

• 6.1Inspect media for any evidence of possible contamination or problems with structure or sterilisation
• 6.2Check useability of selective media by growth of expected organism
• 6.3Check stored stocks at regular intervals for conformance to required standards

7 Maintain work area and equipment to prevent cross-infection and contamination

• 7.1Use personal protective equipment (PPE) and safe work practices to ensure personal safety and that of other laboratory personnel
• 7.2Place disposable and reusable items into relevant receptacles
• 7.3Clean and disinfect work area and equipment after use
• 7.4Transport disposable and reusable contaminated materials to relevant areas for disinfection, sterilisation and cleaning or disposal

What does an assessment tool for MSL973015 need to cover?

To satisfy the Principles of Assessment and Rules of Evidence, an assessment for MSL973015 needs to address all 43 unit components: 7 elements with 26 performance criteria, 4 performance evidence requirements, 13 knowledge evidence requirements, and the foundation skills. A coverage matrix mapping each question and task to these components is what an auditor looks for.

How does Auditori generate an assessment tool for MSL973015?

Auditori pulls the current release of MSL973015 from training.gov.au and generates a complete package: candidate assessment, assessor guide with model answers and observation criteria, and a coverage matrix mapping every component. A suitably qualified person then reviews and approves the draft in a built-in workflow — consistent with ASQA's guidance on AI use in VET — before export as branded PDF and editable Word.

Is the first assessment tool really free?

Yes. Every new account includes one free credit — enough to generate the complete assessment tool for MSL973015 — with no card and no subscription required. After that it's pay-as-you-go per unit.

Can I check my existing MSL973015 assessment instead of generating a new one?

Yes — upload your existing assessment or learner guide and Auditori maps it against every element, performance criterion, PE and KE of MSL973015, showing exactly what's covered and what's missing. Mapping costs a quarter of a credit.