ACMATE506Assist to collect and transfer embryos of mice

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What an assessment for ACMATE506 must cover

47 assessable components: 6 elements (31 performance criteria), 3 performance evidence and 10 knowledge evidence requirements, plus 3 foundation skills. An audit-defensible tool maps every question and task back to these — that mapping is the coverage matrix Auditori generates alongside the assessment.

Elements & performance criteria

1 Prepare for procedures and maintain documentation

  • 1.1Comply with organisational protocols, policies and procedures as set down by the AEC and relevant legislative requirements, including work health and safety, at all times
  • 1.2Identify regulatory and project documentation requirements
  • 1.3Verify project reproductive and breeding program objectives against instructions
  • 1.4Maintain and update records according to organisational policies and procedures

2 Assist to vasectomise males

  • 2.1Prepare equipment and work areas
  • 2.2Select male mice of appropriate age for vasectomy and assess for health status and suitability for surgery
  • 2.3Prepare and verify contingency plans to respond to potential emergencies during and after surgery
  • 2.4Prepare anaesthetics, analgesics and equipment according to instructions or project specifications
  • 2.5Anaesthetise animal under supervision and monitor to ensure surgical anaesthesia is achieved and maintained
  • 2.6Prepare animal for aseptic surgery
  • 2.7Conduct vasectomy and close wounds under supervision
  • 2.8Monitor animal during recovery
  • 2.9Confirm success of vasectomy

3 Assist to collect embryos

  • 3.1Monitor females of appropriate age for stages of oestrus cycle
  • 3.2Identify suitable females and assess health status
  • 3.3Prepare female for super-ovulation according to organisational procedure
  • 3.4Mate female with an entire male
  • 3.5Confirm presence or absence of vaginal plugs
  • 3.6Collect embryos under supervision from the reproductive tract of super-ovulated and mated donor females

4 Manage and manipulate embryos

  • 4.1Hold embryos in a suitable receptacle under optimal temperature and atmospheric conditions to ensure viability
  • 4.2Grade and manipulate embryos
  • 4.3Wash embryos in an aseptic manner for the purpose of re-derivation transfer
  • 4.4Store embryos before transfer
  • 4.5Monitor procedures, materials, equipment and techniques for quality

5 Assist to transfer embryos

  • 5.1Mate females with vasectomised males and check for the presence of vaginal plugs
  • 5.2Prepare embryos for transfer
  • 5.3Under supervision, anaesthetise females for embryo transfer and monitor to ensure surgical anaesthesia is achieved and maintained
  • 5.4Transfer embryos under supervision

6 Monitor success of embryo transfer

  • 6.1Monitor females for pregnancy using appropriate technique
  • 6.2Monitor and record number of pups born to embryos transferred per recipient to determine success of program
  • 6.3Assess health status of pups and recipient after birth and weaning

Performance evidence

  • assisted to collect and transfer embryos of mice on at least two occasions, including:
  • complied with organisational protocols, policies and procedures, including Animal Ethics Committee (AEC), legislative and health and safety requirements, at all times
  • monitored the health and welfare of animals during and after procedures and provided appropriate care.

Knowledge evidence

  • principles and practices for mouse embryo transfer collection, including: - anatomical and physiological features of mice related to vasectomy, embryo collection and transfer - reproductive cycles relating to mouse embryo technology - mouse oogenesis, ovulation, conception and embryonic development - constitution, storage and use of proprietary embryo flushing, holding and culture media - super-ovulation regimens for mice - safe mouse handling techniques, including approved handling methods during the administration of substances and surgical procedures - euthanasia or humane killing techniques for culling mice for embryo collection or if suffering during or after surgery
  • relevant common diseases, injuries and other impacts on mouse health and wellbeing, and characteristics of healthy, sick or distressed mice
  • key requirements of the Australian Code for the Care and Use of Animals for Scientific Purposes relating to the collection and transfer of embryos of mice
  • relevant state or territory legislation and regulations relating to: - practice of veterinary science - workplace health and safety - animal welfare and research, including the Office of the Gene Technology Regulator and its impact on the work in assisting to collect and transfer embryos of mice - use of therapeutic and controlled substances
  • organisational policies and safe work procedures, including: - emergency procedures - hygiene standards – disinfectants and cleaning agents, techniques, equipment and materials - infection control protocols
  • relevant laboratory techniques and procedures, including: - methods used to collect, store and manipulate embryos from mice for re-derivation purposes - methods used to perform embryo transfer procedures in mice - surgery relevant to vasectomy, embryo collection and transfer - cryo preservation and recovery - aseptic techniques
  • relevant anaesthetics, analgesics and other medications used, and methods of administration for mouse surgery
  • applicable industry quality assurance requirements and required documentation
  • biosecurity issues relating to the collection of samples from animals
  • relevant principles of animal welfare and ethics, including the 3Rs (replacement, reduction and refinement) and the 5 Domains.

Foundation skills

  • Reading: Synthesise relevant ideas from several sources, including identifying work requirements from regulatory and project documentation
  • Writing: Note observations during monitoring of animals, including health status of pups and recipient
  • Oral communication: Clarify and discuss instructions about work procedures

Unit content sourced from training.gov.au — © Commonwealth of Australia, licensed under CC BY 4.0. Auditori is not affiliated with the Department of Employment and Workplace Relations.

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Questions about assessing ACMATE506

What does an assessment tool for ACMATE506 need to cover?

To satisfy the Principles of Assessment and Rules of Evidence, an assessment for ACMATE506 needs to address all 47 unit components: 6 elements with 31 performance criteria, 3 performance evidence requirements, 10 knowledge evidence requirements, and the foundation skills. A coverage matrix mapping each question and task to these components is what an auditor looks for.

How does Auditori generate an assessment tool for ACMATE506?

Auditori pulls the current release of ACMATE506 from training.gov.au and generates a complete package: candidate assessment, assessor guide with model answers and observation criteria, and a coverage matrix mapping every component. A suitably qualified person then reviews and approves the draft in a built-in workflow — consistent with ASQA's guidance on AI use in VET — before export as branded PDF and editable Word.

Is the first assessment tool really free?

Yes. Every new account includes one free credit — enough to generate the complete assessment tool for ACMATE506 — with no card and no subscription required. After that it's pay-as-you-go per unit.

Can I check my existing ACMATE506 assessment instead of generating a new one?

Yes — upload your existing assessment or learner guide and Auditori maps it against every element, performance criterion, PE and KE of ACMATE506, showing exactly what's covered and what's missing. Mapping costs a quarter of a credit.

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